Identification of methicillin-resistant strains of Staphylococcus aureus isolated from humans and food sources by use mecA 1 and mecA 2 genes in Pulsed-field gel electrophoresis technique

Staphylococcus aureus causes mastitis in dairy cows, lambs, goats, and skin disorders pigs other animals. S. localized purulent infections that affect soft tissues, bones, organs humans. Using restriction patterns, the researchers want to isolate identify methicillin-resistant (MRSA) strains from cattle They also hope assess their genetic relatedness by comparing mecA1 mecA2 gene sequence discrepancies. Animals (223 strains) people have been used acquire for study (83). The E-test was whether or not bacteria were resistant methicillin. genes identified using pulsed-field gel electrophoresis (PFGE) analyze DNA patterns. results shown. animals men methicillin 32 (14.34 %) 53 (63.8 %), respectively. PFGE determine differences between human veterinary pathology strains. Two of collected discovered be identical; nevertheless, microorganisms recovered humans found significantly similar Both implicated development resistance. MRSA much more significant than those exhibited a high degree heterogeneity. Still, enormous number indistinguishable leads one believe dominant clone is present. When it comes molecular characterization isolates, might regarded as gold standard. Keywords. Animals, Human, MRSA, PFGE, Staphylococcus, mecA